FineTest
SKU:EH0163
Human ICAM-3(Intercellular adhesion molecule 3) ELISA Kit
Human ICAM-3(Intercellular adhesion molecule 3) ELISA Kit
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ICAM-3|CD50
Product Name
Human ICAM-3(Intercellular adhesion molecule 3) ELISA Kit
Alias
ICAM-3 ELISA Kit, CD50 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ICAM-3 ELISA Kit allows for the in vitro quantitative determination of ICAM-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ICAM-3 and the recovery rates were calculated by comparing the measured value to the expected amount of ICAM-3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
95 |
|
EDTA plasma(n=5) |
88-102 |
98 |
|
heparin plasma(n=5) |
85-102 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ICAM-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-104% |
87-101% |
93-103% |
|
EDTA plasma(n=5) |
83-94% |
83-99% |
87-101% |
|
heparin plasma(n=5) |
85-96% |
81-99% |
82-88% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
