FineTest
SKU:EH0160
Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit
Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit
Couldn't load pickup availability
HSP27|Heat Shock Protein 27|HSP-27|HSPB1
Product Name
Human HSP27/HSPB1(Heat Shock Protein 27) ELISA Kit
Alias
HSP27 ELISA Kit, Heat Shock Protein 27 ELISA Kit, HSP-27 ELISA Kit, HSPB1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HSP27/HSPB1 ELISA Kit allows for the in vitro quantitative determination of HSP27/HSPB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HSP27/HSPB1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP27/HSPB1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
98 |
|
EDTA plasma(n=5) |
85-101 |
94 |
|
heparin plasma(n=5) |
85-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP27/HSPB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
88-93% |
88-102% |
|
EDTA plasma(n=5) |
84-97% |
83-98% |
93-101% |
|
heparin plasma(n=5) |
86-94% |
81-91% |
85-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
