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Human RAC1(Ras Related C3 Botulinum Toxin SubstRate 1) ELISA Kit
Human RAC1(Ras Related C3 Botulinum Toxin SubstRate 1) ELISA Kit
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MGC111543|MIG5|TC-25|p21-Rac1|migRation-inducing gene 5|ras-related C3 botulinum toxin substRate 1|rho family|small GTP binding protein Rac1
Product Name
Human RAC1(Ras Related C3 Botulinum Toxin SubstRate 1) ELISA Kit
Alias
MGC111543 ELISA Kit, MIG5 ELISA Kit, TC-25 ELISA Kit, p21-Rac1 ELISA Kit, migRation-inducing gene 5 ELISA Kit, ras-related C3 botulinum toxin substRate 1 ELISA Kit, rho family ELISA Kit, small GTP binding protein Rac1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RAC1 ELISA Kit allows for the in vitro quantitative determination of RAC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RAC1 and the recovery rates were calculated by comparing the measured value to the expected amount of RAC1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
97 |
|
EDTA plasma(n=5) |
87-104 |
93 |
|
heparin plasma(n=5) |
87-103 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
86-102% |
86-96% |
|
EDTA plasma(n=5) |
83-97% |
83-100% |
93-99% |
|
heparin plasma(n=5) |
91-100% |
81-96% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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