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SKU:EH0016
Human MIF(Macrophage migRation inhibitory factor) ELISA Kit
Human MIF(Macrophage migRation inhibitory factor) ELISA Kit
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MIF|Macrophage migRation inhibitory factor|GIFmacrophage migRation inhibitory factor|GLIF|Glycosylation-inhibiting factor|L-dopachrome isomerase|L-dopachrome tautomerase|macrophage migRation inhibitory factor|glycosylation-inhibiting factor|MMIF|Phenylpyruvate tautomerase
Product Name
Human MIF(Macrophage migRation inhibitory factor) ELISA Kit
Alias
MIF ELISA Kit, Macrophage migRation inhibitory factor ELISA Kit, GIFmacrophage migRation inhibitory factor ELISA Kit, GLIF ELISA Kit, Glycosylation-inhibiting factor ELISA Kit, L-dopachrome isomerase ELISA Kit, L-dopachrome tautomerase ELISA Kit, macrophage migRation inhibitory factor ELISA Kit, glycosylation-inhibiting factor ELISA Kit, MMIF ELISA Kit, Phenylpyruvate tautomerase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MIF ELISA Kit allows for the in vitro quantitative determination of MIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MIF and the recovery rates were calculated by comparing the measured value to the expected amount of MIF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-98 |
92 |
|
EDTA plasma(n=5) |
93-101 |
97 |
|
heparin plasma(n=5) |
86-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
85-100% |
90-104% |
|
EDTA plasma(n=5) |
82-93% |
92-101% |
86-94% |
|
heparin plasma(n=5) |
82-96% |
80-97% |
85-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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