FineTest
SKU:ECH0067
Chicken αFP(Alpha-Fetoprotein) ELISA Kit
Chicken αFP(Alpha-Fetoprotein) ELISA Kit
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αFP|Alpha-Fetoprotein|AFP|FETA|HPAFP|oralpha fetal protein
Product Name
Chicken αFP(Alpha-Fetoprotein) ELISA Kit
Alias
αFP ELISA Kit, Alpha-Fetoprotein ELISA Kit, AFP ELISA Kit, FETA ELISA Kit, HPAFP ELISA Kit, oralpha fetal protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
αFP ELISA Kit allows for the in vitro quantitative determination of αFP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Chicken
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of αFP and the recovery rates were calculated by comparing the measured value to the expected amount of αFP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
95 |
|
EDTA plasma(n=5) |
88-102 |
94 |
|
heparin plasma(n=5) |
87-100 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of αFP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-101% |
87-104% |
85-101% |
|
EDTA plasma(n=5) |
83-95% |
82-101% |
83-99% |
|
heparin plasma(n=5) |
84-98% |
86-95% |
82-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
