FineTest
SKU:ECA0020
Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
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TNFα|Tumor Necrosis Factor Alpha|TNF-α|DIF|TNF-alpha|TNFA|TNFSF2
Product Name
Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Alias
TNFα ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Canine
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-100 |
92 |
|
EDTA plasma(n=5) |
90-105 |
96 |
|
heparin plasma(n=5) |
85-105 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-105% |
85-101% |
94-101% |
|
EDTA plasma(n=5) |
87-100% |
88-100% |
83-89% |
|
heparin plasma(n=5) |
81-95% |
80-98% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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