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FineTest

SKU:ECA0020

Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Regular price $500.00 USD
Regular price Sale price $500.00 USD
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size
Sensitivity
Range

TNFα|Tumor Necrosis Factor Alpha|TNF-α|DIF|TNF-alpha|TNFA|TNFSF2

Product Name

Canine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Alias

TNFα ELISA KitTumor Necrosis Factor Alpha ELISA KitTNF-α ELISA KitDIF ELISA KitTNF-alpha ELISA KitTNFA ELISA KitTNFSF2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Species

Canine

UniProt ID

P51742

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-100

92

EDTA plasma(n=5)

90-105

96

heparin plasma(n=5)

85-105

91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

91-105%

85-101%

94-101%

EDTA plasma(n=5)

87-100%

88-100%

83-89%

heparin plasma(n=5)

81-95%

80-98%

90-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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