FineTest
SKU:EB0008
Bovine TNF-α (Tumor necrosis factor alpha) ELISA Kit
Bovine TNF-α (Tumor necrosis factor alpha) ELISA Kit
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TNF-α|DIF|TNF-alpha|TNFA|TNFSF2
Product Name
Bovine TNF-α(Tumor necrosis factor alpha) ELISA Kit
Alias
TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-Α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Bovine
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TNF-Α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-Α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-104 |
97 |
|
EDTA plasma(n=5) |
86-98 |
91 |
|
heparin plasma(n=5) |
87-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-Α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
85-103% |
86-100% |
|
EDTA plasma(n=5) |
83-101% |
85-97% |
83-97% |
|
heparin plasma(n=5) |
84-100% |
87-100% |
80-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
