Recombinant Human GLO1

Product Name

Recombinant Human GLO1

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

2-184

Mol. Weight

22.5 KDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to catalyze the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced glutathione

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase, Lactoylglutathione lyase, LGUL_HUMAN, Methylglyoxalase, S D lactoylglutathione methylglyoxal lyase, S-D-lactoylglutathione methylglyoxal lyase