Recombinant Human IL-4

Product Name

Recombinant Human IL-4

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

25-153

Mol. Weight

35.6 kDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.2-1 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

B cell growth factor 1, B cell IgG differentiation factor, B Cell Stimulatory Factor 1, B-cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin, BSF-1, BSF1, IGG1 induction factor, IL 4, IL-4, IL4, IL4_HUMAN, Il4e12, Interleukin 4, Interleukin 4 variant 2, Interleukin 4, isoform 1