Recombinant SARS-CoV-2 Spike Glycoprotein

Product Name

Recombinant SARS-CoV-2 Spike Glycoprotein

Size

50μg, 200μg, 1mg

Species

Coronavirus

Host

HEK293 cells

Expression Region

16-1213

Mol. Weight

180 KDa

Tags

C-terminal His-Avi Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE2 (Catalog # P2955).

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

Spike glycoprotein, S glycoprotein