Recombinant Human/Mouse/Rat Irisin

Product Name

Recombinant Human/Mouse/Rat Irisin

Size

50μg, 200μg, 1mg

Species

Human/Mouse/Rat

Host

HEK293 cells

Expression Region

32-143

Mol. Weight

17-25kDa

Tags

C-terminal His Tag

Purity

Greater than 90% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to induce p38 MAPK activation in 3T3‑L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

Fibronectin type III domain containing 5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat containing protein 2, Fibronectin type III repeat-containing protein 2, FNDC 5, Fndc5, FNDC5_HUMAN, FRCP2, irisin