Recombinant Human PF4

Product Name

Recombinant Human PF4

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

31-101

Mol. Weight

28.4 kDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 3-15 µg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, chemokine ligand 4, CXCL 4, CXCL4, Iroplact, MGC138298, Oncostatin A, Oncostatin-A, OncostatinA, PF 4, PF-4, PF4, Pf4a, Platelet factor 4, PLF4_HUMAN, SCYB 4, SCYB4, short form