Recombinant Mouse GM-CSF

Product Name

Recombinant Mouse GM-CSF

Size

50μg, 200μg, 1mg

Species

Mouse

Host

HEK293 cells

Expression Region

18-141

Mol. Weight

23 kDa

Tags

C-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 20-80 pg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF2, CSF-2, GMCSF, GM-CSF, GMCSFgranulocyte-macrophage colony-stimulating factor, granulocyte-macrophage colony stimulating factor, MGC131935, MGC138897, Molgramostim, molgramostin, Sargramostim