Recombinant Human UBC4

Product Name

Recombinant Human UBC4

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

1-147

Mol. Weight

37.4 kDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

PUBC 1, PUBC1, UB2D2_HUMAN, UBC 4, UBC 4/5, UBC4, UBC4/5, UBC4/5 homolog yeast, UBCH 5B, UBCH5B, UBE2D2, Ubiquitin carrier protein, Ubiquitin carrier protein D2, Ubiquitin conjugating enzyme E2 17 kDa 2, Ubiquitin conjugating enzyme E2 D2, Ubiquitin conjugating enzyme E2D 2, Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5)