anti- HA-Tag antibody

Product Name

HA-Tag antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

IMMUNOGEN INFORMATION

Immunogen

HA tag

APPLICATION

Tested Application

ELISA, IP, WB, IF

Recommended Dilution

WB: 1:5000-1:50000; IP: 1:2000-1:20000; IF: 1:10-1:100

IMAGES

IP Result of anti-HA-tag (IP: FNab03762, 5ug; Detection: FNab03762 1:5000) with Transfected HEK-293 cells lysate 400ug.

HA-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03762(HA tag Antibody) at dilution of 1:20000