anti- SOD2 antibody

anti- SOD2 antibody

Product Name

SOD2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

SOD2(superoxide dismutase 2, mitochondrial) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

IMMUNOGEN INFORMATION

Immunogen

superoxide dismutase 2, mitochondrial

Synonyms

Superoxide dismutase [Mn], mitochondrial(EC:1.15.1.1),SOD2

Observed MW

25 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IHC, IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P04179

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08104(SOD2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of HeLa cells using FNab08104 (SOD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-SOD2 (IP:FNab08104, 4ug; Detection:FNab08104 1:1000) with mouse brain tissue lysate 4000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab08104(SOD2 antibody) at dilution of 1:1000