anti- SPOP antibody

anti- SPOP antibody

Product Name

SPOP antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of a cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

IMMUNOGEN INFORMATION

Immunogen

speckle-type POZ protein

Synonyms

HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2

Observed MW

42 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:500; IP : 1:500-1:1000

UNIPROT INFORMATION

UniProt ID

O43791

IMAGES

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab08189( SPOP Antibody) at dilution of 1:200

IP Result of anti-SPOP (IP:FNab08189, 3ug; Detection:FNab08189 1:500) with HeLa cell lysate 3000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab08189 (SPOP antibody) at dilution of 1:500