anti- STAT3 antibody

anti- STAT3 antibody

Product Name

STAT3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

IMMUNOGEN INFORMATION

Immunogen

signal transducer and activator of transcription 3 (acute-phase response factor)

Synonyms

Acute phase response factor, APRF, HIES, STAT3

Observed MW

85 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:200 - 1:500; IHC: 1:50 - 1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P40763

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer slide using FNab08298( STAT3 Antibody) at dilution of 1:50

Immunofluorescent analysis of HeLa cells using FNab08298 ( STAT3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

HeLa cells were subjected to SDS PAGE followed by western blot with FNab08298(STAT3 antibody) at dilution of 1:300