anti- STIM1 antibody

anti- STIM1 antibody

Product Name

STIM1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. This antibody is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

IMMUNOGEN INFORMATION

Immunogen

stromal interaction molecule 1

Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1

Observed MW

80kd

APPLICATION

Tested Application

ELISA, WB, IP, IHC, IF, FC

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q13586

IMAGES

Immunohistochemistry of paraffin-embedded human ovary tumor using FNab08320(STIM1 antibody) at dilution of 1:50

IP Result of anti-STIM1 (IP: FNab08320, 3ug; Detection: FNab08320 1:1000) with Raji cells lysate 4000ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08320(STIM1 antibody) at dilution of 1:300