anti- STT3A antibody

anti- STT3A antibody

Product Name

STT3A antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Catalytic subunit of the N-oligosaccharyl transferase(OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum(ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.

IMMUNOGEN INFORMATION

Immunogen

STT3, subunit of the oligosaccharyltransferase complex, homolog A

Synonyms

B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC

Observed MW

100 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:1000-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P46977

IMAGES

Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab08355(STT3A antibody) at dilution of 1:50

Immunofluorescent analysis of HEK-293 cells using FNab08355 (STT3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab08355(STT3A Antibody) at dilution of 1:2000