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FNab08425

anti- Synaptotagmin-1 antibody

anti- Synaptotagmin-1 antibody

$260.00 USD
$260.00 USD
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Size
Host
Clonality

Product Name

Synaptotagmin-1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis(PMID: 8058779). SYT1(synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue(PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse(PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth(PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

IMMUNOGEN INFORMATION

Immunogen

synaptotagmin I

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, SYT, SYT1, SytI

Observed MW

65 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P21579

IMAGES

 

Immunohistochemistry of paraffin-embedded human liver cancer using FNab08425(SYT1 antibody) at dilution of 1:100
Immunofluorescent analysis of ( -20℃ Ethanol) fixed HepG2 cells using FNab08425 (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IP Result of anti-Synaptotagmin-1 (IP:FNab08425, 4ug; Detection:FNab08425 1:500) with mouse testis tissue lysate 4000ug.
human liver tissue were subjected to SDS PAGE followed by western blot with FNab08425(SYT1 antibody) at dilution of 1:1000

 

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