anti- VPS4A antibody

Product Name

VPS4A antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Involved in late steps of the endosomal multivesicular bodies(MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles(ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding(HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission(PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan(PubMed:22660413).

IMMUNOGEN INFORMATION

Immunogen

vacuolar protein sorting 4 homolog A

Synonyms

FLJ22197, hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A

Observed MW

49 kDa

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:200-1:1000

UNIPROT INFORMATION

UniProt ID

Q9UN37

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