anti- WTAP antibody

PRODUCT INFORMATION

Product Name

WTAP antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).

IMMUNOGEN INFORMATION

Immunogen

Wilms tumor 1 associated protein

Synonyms

DKFZp686F20131, Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

Observed MW

49 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:1000-1:10000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q15007

Immunohistochemistry of paraffin-embedded human cervical cancer using FNab09530(WTAP antibody) at dilution of 1:50

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09530(WTAP Antibody) at dilution of 1:2000