anti- YTHDF1 antibody

PRODUCT INFORMATION

Product Name

YTHDF1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Specifically recognizes and binds N6-methyladenosine(m6A)-containing mRNAs, and promotes mRNA translation efficiency(PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3(EIF3A or EIF3B) to facilitate translation initiation(PubMed:26046440).

IMMUNOGEN INFORMATION

Immunogen

YTH domain family, member 1

Synonyms

C20orf21, DACA 1, FLJ20391, YTH domain family protein 1, YTH domain family, member 1, YTHDF1

Observed MW

62 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9BYJ9

Immunohistochemistry of paraffin-embedded human brain using FNab09572(YTHDF1 antibody) at dilution of 1:100

IP Result of anti-YTHDF1 (IP:FNab09572, 4ug; Detection:FNab09572 1:1000) with mouse brain tissue lysate 2640ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab09572(YTHDF1 antibody) at dilution of 1:500