anti- E-cadherin antibody

Investigate the intricacies of cell-cell adhesion and tissue organization with our meticulously crafted Anti-E-cadherin Antibody, now available in a 100µg size. E-cadherin is a critical protein involved in adherens junctions, playing a pivotal role in tissue integrity and embryonic development.

This antibody is rigorously tested for specificity, ensuring that your research on developmental biology, cancer metastasis, and epithelial-mesenchymal transition is supported by a reliable tool.

E-cadherin's significance in cell adhesion and its role in various physiological and pathological processes have made it a subject of significant research interest. Whether your work is centered on developmental biology, cancer biology, or tissue engineering, this antibody is an invaluable addition to your toolkit.

Product Name

E-cadherin antibody

Size

100µg

Form

liquid

Purification

protein A+G purified

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2b

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.

IMMUNOGEN INFORMATION

Immunogen

cadherin 1, type 1, E-cadherin (epithelial)

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, LCAM, UVO, Uvomorulin

Observed MW

130 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:1000-1:5000; IHC: 1:500-1:2000; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P12830

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab10112( E-cadherin Antibody) at dilution of 1:1000

Immunofluorescent analysis of A431 cells using FNab10112( E-cadherin Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

A431 cells were subjected to SDS PAGE followed by western blot with FNab10112(E-cadherin antibody) at dilution of 1:3000