anti- γ-H2AX antibody

Product Name

γ-H2AX antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.

IMMUNOGEN INFORMATION

Immunogen

A synthetic phosphorylated peptide around S139 of human H2AX

Synonyms

gamma H2A.X,γH2AX, Phospho-H2AX-S139

Observed MW

17 kDa

APPLICATION

Tested Application

WB, IHC, IF

Recommended Dilution

WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100

UNIPROT INFORMATION

UniProt ID

P16104

IMAGES

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using FNab10441(γ-H2AX Antibody) at dilution of 1:100

Immunofluorescent analysis of HeLa cells using FNab10441 (γ-H2AX antibody) at dilution of 1:100. HeLa cells were treated by UV for 15-30 minutes at RT.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab10441(γ-H2AX Antibody) at dilution of 1:1000. NIH/3T3 cells were treated by UV for 15-30 minutes.