anti- EIF2AK1 antibody

Dive into the realm of EIF2AK1 signaling pathways with our Anti-EIF2AK1 Antibody, now available in a 100µg size. EIF2AK1, also known as heme-regulated inhibitor, is a crucial regulator of protein synthesis in response to stress signals.

This antibody is rigorously validated for specificity, ensuring that your research on EIF2AK1's roles in translational control, stress response, and its implications in various cellular processes is supported by a reliable tool.

EIF2AK1's significance in orchestrating cellular stress responses and its involvement in diseases have made it a subject of substantial research interest. Whether your research focuses on integrated stress responses, translational regulation, or disease mechanisms, this antibody is an essential addition to your toolkit.

Order your 100µg vial today and embark on a journey to unravel EIF2AK1's functions and their impact on cellular processes.

Product Name

EIF2AK1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells(RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions(By similarity).

IMMUNOGEN INFORMATION

Immunogen

eukaryotic translation initiation factor 2-alpha kinase 1

Synonyms

EIF2AK1, HCR, HRI, eukaryotic translation initiation factor 2-alpha kinase 1

Observed MW

60-71 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

Q9BQI3

IMAGES

Immunohistochemical of paraffin-embedded human colon using FNab10450(EIF2AK1 antibody) at dilution of 1:100

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab10450(EIF2AK1 antibody) at dilution of 1:1000