anti-GPI antibody

Product Name

GPI antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.

IMMUNOGEN INFORMATION

Immunogen

Glucose-6-phosphate isomerase

Synonyms

GPI, AMF, GNPI, NLK, PGI

Observed MW

55-64 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:2000-1:20000; IHC: 1:200-1:1000; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P06744

IMAGES

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab10496(GPI antibody) at dilution of 1:500

HeLa cells were subjected to SDS PAGE followed by western blot with FNab10496(GPI antibody) at dilution of 1:5000