anti- Nestin antibody

Product Name

Nestin antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

IMMUNOGEN INFORMATION

Immunogen

nestin

Synonyms

NES, Nestin

Observed MW

170 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:500-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P48681

IMAGES

Immunohistochemistry of paraffin-embedded mouse brain using FNab10534(NES antibody) at dilution of 1:50

Immunofluorescent analysis of SH-SY5Y cells using FNab10534(NES antibody) at dilution of 1:50.

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab10534(NES antibody) at dilution of 1:1000