FineTest
SKU:EGP 24.00
Guinea pig IL-12(Interleukin 12) ELISA Kit
Guinea pig IL-12(Interleukin 12) ELISA Kit
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Product Name
Guinea pig IL-12(Interleukin 12) ELISA Kit
Alias
IL-12 ELISA Kit, Cytotoxic lymphocyte matuRation factor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Guinea pig
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
96 |
|
EDTA plasma(n=5) |
86-104 |
96 |
|
heparin plasma(n=5) |
92-100 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
89-104% |
85-102% |
|
EDTA plasma(n=5) |
83-92% |
88-97% |
83-100% |
|
heparin plasma(n=5) |
81-94% |
80-99% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
