FineTest
SKU:EH8100
Human E2F1(Transcription factor E2F1) ELISA Kit
Human E2F1(Transcription factor E2F1) ELISA Kit
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Product Name
Human E2F1(Transcription factor E2F1) ELISA Kit
Alias
Transcription factor E2F1 ELISA Kit, E2F-1 ELISA Kit, PBR3 ELISA Kit, Retinoblastoma-associated protein 1 ELISA Kit, RBAP-1 ELISA Kit, Retinoblastoma-binding protein 3 ELISA Kit, RBBP-3 ELISA Kit, pRB-binding protein E2F-1 ELISA Kit, RBBP3 ELISA Kit, E2F1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
E2F1 ELISA Kit allows for the in vitro quantitative determination of E2F1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of E2F1 and the recovery rates were calculated by comparing the measured value to the expected amount of E2F1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-100 |
92 |
|
EDTA plasma(n=5) |
91-99 |
95 |
|
heparin plasma(n=5) |
88-95 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2F1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
85-94% |
90-105% |
|
EDTA plasma(n=5) |
83-99% |
84-87% |
83-100% |
|
heparin plasma(n=5) |
80-100% |
81-100% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
