FineTest
SKU:EH4508
Human NPNT(Nephronectin) ELISA Kit
Human NPNT(Nephronectin) ELISA Kit
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Product Name
Human NPNT (Nephronectin) ELISA Kit
Alias
Nephronectin ELISA Kit, Preosteoblast EGF-like repeat protein with MAM domain ELISA Kit, Protein EGFL6-like ELISA Kit, EGFL6L ELISA Kit, POEM ELISA Kit, UNQ295 ELISA Kit, PRO334 ELISA Kit, NPNT ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NPNT ELISA Kit allows for the in vitro quantitative determination of NPNT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NPNT and the recovery rates were calculated by comparing the measured value to the expected amount of NPNT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
92 |
|
EDTA plasma(n=5) |
85-105 |
93 |
|
heparin plasma(n=5) |
85-101 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPNT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
90-101% |
85-100% |
|
EDTA plasma(n=5) |
85-98% |
88-101% |
88-100% |
|
heparin plasma(n=5) |
93-99% |
84-94% |
80-89% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
