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EH12550

Human SPHK2(Sphingosine kinase 2) ELISA Kit

Human SPHK2(Sphingosine kinase 2) ELISA Kit

$400.00 USD
$400.00 USD
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Size
Sensitivity
Range

Product Name

Human SPHK2(Sphingosine kinase 2) ELISA Kit

Alias

Sphingosine kinase 2 ELISA Kit, SK 2 ELISA Kit, SPK 2 ELISA Kit, SK2 ELISA Kit, SPHK2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

SPHK2 ELISA Kit allows for the in vitro quantitative determination of SPHK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Human

UniProt ID

Q9NRA0

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SPHK2 and the recovery rates were calculated by comparing the measured value to the expected amount of SPHK2 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-95

90

EDTA plasma(n=5)

87-101

94

heparin plasma(n=5)

86-103

97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPHK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-102%

96-102%

94-102%

EDTA plasma(n=5)

85-101%

86-94%

87-99%

heparin plasma(n=5)

86-98%

88-99%

85-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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