FineTest
SKU:EH15355
Human SPTA1(Spectrin alpha chain, erythrocytic 1) ELISA Kit
Human SPTA1(Spectrin alpha chain, erythrocytic 1) ELISA Kit
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Product Name
Human SPTA1 (Spectrin alpha chain, erythrocytic 1) ELISA Kit
Alias
Spectrin alpha chain, erythrocytic 1 ELISA Kit, Erythroid alpha-spectrin ELISA Kit, SPTA ELISA Kit, SPTA1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SPTA1 ELISA Kit allows for the in vitro quantitative determination of SPTA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SPTA1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPTA1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-103 |
97 |
|
EDTA plasma(n=5) |
89-99 |
95 |
|
heparin plasma(n=5) |
85-99 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPTA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
96-105% |
90-103% |
88-100% |
|
EDTA plasma(n=5) |
83-100% |
83-100% |
82-101% |
|
heparin plasma(n=5) |
80-100% |
80-99% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
