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EH6661

Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

$400.00 USD
$400.00 USD
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Size
Sensitivity
Range

Product Name

Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

Alias

2-oxoisovalerate dehydrogenase subunit beta, mitochondrial ELISA Kit, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain ELISA Kit, BCKDE1B ELISA Kit, BCKDH E1-beta ELISA Kit, BCKDHB ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

BCKDHB ELISA Kit allows for the in vitro quantitative determination of BCKDHB concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Species

Human

UniProt ID

P21953

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BCKDHB and the recovery rates were calculated by comparing the measured value to the expected amount of BCKDHB in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-105

95

EDTA plasma(n=5)

88-100

95

heparin plasma(n=5)

87-102

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCKDHB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-105%

87-105%

85-102%

EDTA plasma(n=5)

87-97%

88-96%

83-97%

heparin plasma(n=5)

84-99%

87-93%

82-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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