FineTest
SKU:EH8494
Human FEN1(Flap endonuclease 1) ELISA Kit
Human FEN1(Flap endonuclease 1) ELISA Kit
Couldn't load pickup availability
Product Name
Human FEN1(Flap endonuclease 1) ELISA Kit
Alias
Flap endonuclease 1 ELISA Kit, FEN-1 ELISA Kit, DNase IV ELISA Kit, Flap structure-specific endonuclease 1 ELISA Kit, Maturation factor 1 ELISA Kit, MF1 ELISA Kit, hFEN-1 ELISA Kit, FEN1 ELISA Kit, RAD2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FEN1 ELISA Kit allows for the in vitro quantitative determination of FEN1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FEN1 and the recovery rates were calculated by comparing the measured value to the expected amount of FEN1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-98 |
93 |
|
EDTA plasma(n=5) |
86-100 |
92 |
|
heparin plasma(n=5) |
90-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FEN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
87-101% |
94-103% |
|
EDTA plasma(n=5) |
83-99% |
89-101% |
82-99% |
|
heparin plasma(n=5) |
81-96% |
82-100% |
81-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
