FineTest
SKU:EM0109-CM
Mouse IL-1b(Interleukin 1 Beta) ELISA Kit
Mouse IL-1b(Interleukin 1 Beta) ELISA Kit
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Product Name
Mouse IL-1b(Interleukin 1 Beta) ELISA Kit
Alias
IL-1b ELISA Kit, Interleukin 1 Beta ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1b ELISA Kit allows for the in vitro quantitative determination of IL-1b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 5pg/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1b in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
93 |
|
EDTA plasma(n=5) |
85-101 |
94 |
|
heparin plasma(n=5) |
87-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-101% |
87-100% |
88-103% |
|
EDTA plasma(n=5) |
82-98% |
86-101% |
82-100% |
|
heparin plasma(n=5) |
83-99% |
80-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
