FineTest
SKU:ER1910
Rat PI3Kca(PI3K-catalitic subunit alpha) ELISA Kit
Rat PI3Kca(PI3K-catalitic subunit alpha) ELISA Kit
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Product Name
Rat PI3Kca(PI3K-catalitic subunit alpha) ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PI3Kca ELISA Kit allows for the in vitro quantitative determination of PI3Kca concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PI3Kca and the recovery rates were calculated by comparing the measured value to the expected amount of PI3Kca in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
95 |
|
EDTA plasma(n=5) |
95-101 |
98 |
|
heparin plasma(n=5) |
92-100 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PI3Kca and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-102% |
86-98% |
91-104% |
|
EDTA plasma(n=5) |
84-101% |
83-101% |
82-101% |
|
heparin plasma(n=5) |
82-98% |
82-88% |
84-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
