FineTest
SKU:EH4483
Human GPR176(G-protein coupled receptor 176)ELISA Kit
Human GPR176(G-protein coupled receptor 176)ELISA Kit
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Product Name
Human GPR176(G-protein coupled receptor 176)ELISA Kit
Alias
GPR176 ELISA Kit, G-protein coupled receptor 176 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GPR176ELISA Kit allows for the in vitro quantitative determination of GPR176 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GPR176 and the recovery rates were calculated by comparing the measured value to the expected amount of GPR176 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-104 |
99 |
|
EDTA plasma(n=5) |
93-105 |
99 |
|
heparin plasma(n=5) |
95-103 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPR176 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
85-98% |
87-104% |
|
EDTA plasma(n=5) |
84-96% |
86-96% |
88-100% |
|
heparin plasma(n=5) |
93-100% |
81-100% |
87-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
