FineTest
SKU:EM7199
Mouse H3f3b(Histone H3.3) ELISA Kit
Mouse H3f3b(Histone H3.3) ELISA Kit
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Product Name
Mouse H3f3b (Histone H3.3) ELISA Kit
Alias
Histone H3.3 ELISA Kit, H3-3a ELISA Kit, H3.3a ELISA Kit, H3f3a ELISA Kit, H3-3b ELISA Kit, H3.3b ELISA Kit, H3f3b ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
H3f3b ELISA Kit allows for the in vitro quantitative determination of H3f3b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of H3f3b and the recovery rates were calculated by comparing the measured value to the expected amount of H3f3b in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-104 |
96 |
|
EDTA plasma(n=5) |
89-99 |
93 |
|
heparin plasma(n=5) |
92-105 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H3f3b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
87-104% |
86-96% |
|
EDTA plasma(n=5) |
83-85% |
83-96% |
84-101% |
|
heparin plasma(n=5) |
81-90% |
83-100% |
85-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
