FineTest
SKU:EH6283
Human AMPH(Amphiphysin) ELISA Kit
Human AMPH(Amphiphysin) ELISA Kit
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Product Name
Human AMPH(Amphiphysin) ELISA Kit
Alias
Amphiphysin ELISA Kit, AMPH ELISA Kit, AMPH1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AMPH ELISA Kit allows for the in vitro quantitative determination of AMPH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AMPH and the recovery rates were calculated by comparing the measured value to the expected amount of AMPH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-99 |
94 |
|
EDTA plasma(n=5) |
92-105 |
97 |
|
heparin plasma(n=5) |
93-103 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
85-97% |
93-104% |
|
EDTA plasma(n=5) |
84-93% |
82-101% |
82-98% |
|
heparin plasma(n=5) |
83-98% |
86-97% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
