FineTest
SKU:EH10983
Human PDE9A(High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) ELISA Kit
Human PDE9A(High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) ELISA Kit
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Product Name
Human PDE9A(High affinity cGMP-specific 3′,5′-cyclic phosphodiesterase 9A) ELISA Kit
Alias
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A ELISA Kit, PDE9A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDE9A ELISA Kit allows for the in vitro quantitative determination of PDE9A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDE9A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE9A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
97 |
|
EDTA plasma(n=5) |
91-99 |
95 |
|
heparin plasma(n=5) |
93-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE9A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-100% |
90-99% |
88-102% |
|
EDTA plasma(n=5) |
85-100% |
84-99% |
88-101% |
|
heparin plasma(n=5) |
88-96% |
84-92% |
90-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
