FineTest
SKU:EH12921
Human TFEB(Transcription factor EB) ELISA Kit
Human TFEB(Transcription factor EB) ELISA Kit
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Product Name
Human TFEB(Transcription factor EB)ELISA Kit
Alias
Transcription factor EB ELISA Kit, Class E basic helix-loop-helix protein 35 ELISA Kit, bHLHe35 ELISA Kit, TFEB ELISA Kit, BHLHE35 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TFEBELISA Kit allows for the in vitro quantitative determination of TFEB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TFEB and the recovery rates were calculated by comparing the measured value to the expected amount of TFEB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-103 |
99 |
|
EDTA plasma(n=5) |
92-102 |
95 |
|
heparin plasma(n=5) |
89-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFEB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
86-99% |
89-102% |
|
EDTA plasma(n=5) |
83-100% |
84-100% |
82-98% |
|
heparin plasma(n=5) |
81-98% |
81-99% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
