FineTest
SKU:EM1987
Mouse H1-4(Histone H1.4) ELISA Kit
Mouse H1-4(Histone H1.4) ELISA Kit
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Product Name
Mouse H1-4 (Histone H1.4) ELISA Kit
Alias
Histone H1.4 ELISA Kit, H1 VAR.2 ELISA Kit, H1e ELISA Kit, H1-4 ELISA Kit, H1f4 ELISA Kit, Hist1h1e ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
H1-4 ELISA Kit allows for the in vitro quantitative determination of H1-4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of H1-4 and the recovery rates were calculated by comparing the measured value to the expected amount of H1-4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-103 |
97 |
|
EDTA plasma(n=5) |
83-103 |
93 |
|
heparin plasma(n=5) |
85-105 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H1-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
94-102% |
80-101% |
87-96% |
|
EDTA plasma(n=5) |
90-101% |
90-101% |
93-101% |
|
heparin plasma(n=5) |
90-99% |
90-98% |
91-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
