FineTest
SKU:EU2603
S1P(Sphingosine 1 Phosphate) ELISA Kit
S1P(Sphingosine 1 Phosphate) ELISA Kit
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Product Name
S1P(Sphingosine 1 Phosphate) ELISA Kit
Alias
S1P ELISA Kit, Sphingosine 1 Phosphate ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
S1P ELISA Kit allows for the in vitro quantitative determination of SIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.13-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIP and the recovery rates were calculated by comparing the measured value to the expected amount of SIP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
97-104 |
100 |
|
EDTA plasma(n=5) |
95-98 |
96 |
|
heparin plasma(n=5) |
96-102 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
95-105% |
85-100% |
|
EDTA plasma(n=5) |
89-101% |
89-94% |
89-99% |
|
heparin plasma(n=5) |
93-100% |
90-98% |
90-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
