FineTest
SKU:ER6559
Rat Gls(Glutaminase kidney isoform, mitochondrial) ELISA Kit
Rat Gls(Glutaminase kidney isoform, mitochondrial) ELISA Kit
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Product Name
Rat Gls (Glutaminase kidney isoform, mitochondrial) ELISA Kit
Alias
Glutaminase kidney isoform, mitochondrial ELISA Kit, GLS ELISA Kit, K-glutaminase ELISA Kit, L-glutamine amidohydrolase ELISA Kit, Glutaminase kidney isoform, mitochondrial 68 kDa chain ELISA Kit, Glutaminase kidney isoform, mitochondrial 65 kDa chain ELISA Kit, Gls ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gls ELISA Kit allows for the in vitro quantitative determination of Gls concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Gls and the recovery rates were calculated by comparing the measured value to the expected amount of Gls in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
84-103 |
93 |
|
EDTA plasma(n=5) |
84-104 |
93 |
|
heparin plasma(n=5) |
85-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gls and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
87-105% |
85-104% |
|
EDTA plasma(n=5) |
90-101% |
92-101% |
91-101% |
|
heparin plasma(n=5) |
93-100% |
90-99% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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