FineTest
SKU:EH4517
Human VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation) ELISA Kit
Human VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation) ELISA Kit
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Product Name
Human VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation) ELISA Kit
Alias
V-type immunoglobulin domain-containing suppressor of T-cell activation ELISA Kit, Platelet receptor Gi24 ELISA Kit, Stress-induced secreted protein-1 ELISA Kit, Sisp-1 ELISA Kit, V-set domain-containing immunoregulatory receptor ELISA Kit, V-set immunoregulatory receptor ELISA Kit, C10orf54 ELISA Kit, SISP1 ELISA Kit, VISTA ELISA Kit, PP2135 ELISA Kit, UNQ730 ELISA Kit, PRO1412 ELISA Kit, VSIR ELISA Kit, VISTA ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VISTA ELISA Kit allows for the in vitro quantitative determination of VISTA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VISTA and the recovery rates were calculated by comparing the measured value to the expected amount of VISTA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
83-101 |
91 |
|
EDTA plasma(n=5) |
82-98 |
93 |
|
heparin plasma(n=5) |
90-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VISTA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
80-102% |
80-97% |
85-99% |
|
EDTA plasma(n=5) |
92-98% |
91-101% |
92-100% |
|
heparin plasma(n=5) |
93-99% |
92-100% |
93-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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