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SKU:EH4530
Human RNF-111(E3 ubiquitin-protein ligase Arkadia)ELISA Kit
Human RNF-111(E3 ubiquitin-protein ligase Arkadia)ELISA Kit
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Product Name
Human RNF-111 (E3 ubiquitin-protein ligase Arkadia)ELISA Kit
Alias
E3 ubiquitin-protein ligase Arkadia ELISA Kit, RING finger protein 111 ELISA Kit, hRNF111 ELISA Kit, RING-type E3 ubiquitin transferase Arkadia ELISA Kit, RNF111 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RNF-111 ELISA Kit allows for the in vitro quantitative determination of RNF-111 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RNF-111 and the recovery rates were calculated by comparing the measured value to the expected amount of RNF-111 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
81-104 |
97 |
|
EDTA plasma(n=5) |
84-105 |
97 |
|
heparin plasma(n=5) |
82-94 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RNF-111 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-98% |
85-103% |
87-98% |
|
EDTA plasma(n=5) |
93-101% |
91-99% |
96-101% |
|
heparin plasma(n=5) |
93-99% |
90-100% |
92-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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