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EM6007

Mouse Bckdha(2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

Mouse Bckdha(2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

$400.00 USD
$400.00 USD
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Size
Sensitivity
Range

Product Name

Mouse Bckdha (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

Alias

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial ELISA Kit, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain ELISA Kit, BCKDE1A ELISA Kit, BCKDH E1-alpha ELISA Kit, Bckdha ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Bckdha ELISA Kit allows for the in vitro quantitative determination of Bckdha concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Mouse

UniProt ID

P50136

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Bckdha and the recovery rates were calculated by comparing the measured value to the expected amount of Bckdha in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

90-104

97

EDTA plasma(n=5)

89-105

96

heparin plasma(n=5)

89-100

92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bckdha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

81-105%

80-96%

82-104%

EDTA plasma(n=5)

93-100%

90-99%

91-96%

heparin plasma(n=5)

91-97%

93-100%

91-99%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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