FineTest
SKU:EM1989
Mouse Bckdhb(2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit
Mouse Bckdhb(2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit
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Product Name
Mouse Bckdhb (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit
Alias
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial ELISA Kit, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain ELISA Kit, BCKDE1B ELISA Kit, BCKDH E1-beta ELISA Kit, Bckdhb ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Bckdhb ELISA Kit allows for the in vitro quantitative determination of Bckdhb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Bckdhb and the recovery rates were calculated by comparing the measured value to the expected amount of Bckdhb in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
81-105 |
92 |
|
EDTA plasma(n=5) |
82-104 |
95 |
|
heparin plasma(n=5) |
84-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bckdhb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
85-99% |
82-104% |
|
EDTA plasma(n=5) |
92-99% |
91-101% |
91-98% |
|
heparin plasma(n=5) |
92-99% |
91-97% |
91-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
